ABSTRACT
BACKGROUND: Metastatic melanoma has a high mortality rate and poor survival. This is associated with efficient metastatic colonization, but the underlying mechanisms remain elusive. Communication between cancer stem cells (CSCs) and cancer cells plays an important role in metastatic dissemination. Whether cancer stem cells can alter the metastatic properties of non-CSC cells; and whether exosomal crosstalk can mediate such interaction, have not been demonstrated in melanoma prior to this report. RESULTS: The results revealed that exosomes secreted by highly metastatic melanoma CSCs (OL-SCs) promoted the invasiveness of the low metastatic melanoma cells (OL) and accelerated metastatic progression. miR-1268a was up-regulated in cells and exosomes of OL-SCs. Moreover, OL-SCs-derived exosomal miR-1268a, upon taking up by OL cells, promoted the metastatic colonization ability of OL cells in vitro and in vivo. In addition, the pro-metastatic activity of exosomal miR-1268a is achieved through inhibition of autophagy. CONCLUSION: Our study demonstrates that OL cells can acquire the "metastatic ability" from OL-SCs cells. OL-SCs cells achieves this goal by utilizing its exosomes to deliver functional miRNAs, such as miR-1268a, to the targeted OL cells which in turn augments metastatic colonization by inactivating the autophagy pathway in OL cells.
Subject(s)
Humans , MicroRNAs/metabolism , Exosomes/metabolism , Melanoma/metabolism , Autophagy , Stem Cells , Cell Line, Tumor , Neoplasm MetastasisABSTRACT
Objective@#To explore the effect of anterior alveolar bone reconstruction achieved through autogenous block bone from the wisdom teeth extraction zone in onlay grafting technique. @*Methods@#The 20 patients with bone defect of anterior teeth area were selected. They all had impacted teeth with no infection factors of pericoronitis to be removed. They were treated with autogenous block bone grafts from wisdom teeth extraction zone in onlay grafting technique respectively. The changes of thickness and the height of the bone measured from CBCT before and after 6 months of the surgery were carefully compared. Questionnaire from the patients opinion of acceptance was also be collected. @*Results@# Acceptance questionnaire showed an average score of 55 points (60 full score). Among them, 75% of paitients showed totally supportion and 25% showed basically supportion. The thickness was 6.73 ± 0.28 mm after the surgery compared with 2.26 ± 0.57 mm before the surgery which illustrated a significant difference (F = 6.32, P<0.001). The height didn't change obviously before 14.32 ± 0.31 mm and after 14.56 ± 0.35 mm the surgery. The technique of using autogenous block bone from the wisdom teeth extraction zone improved the compliance of patients with surgery. It can provide good osteogenesis effect and solve other oral diseases at the same time. @*Conclusion@#The Onlay grafting technique which using autogenous block bone from the wisdom teeth extraction zone was a simple and effective techniques for anterior alveolar bone augmentation.